Citation
Yasin, Yamin and Basri, Mahiran and Ahmad, Faujan and Salleh, Abu Bakar
(2008)
Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester.
Journal of Chemical Technology and Biotechnology, 83 (5).
pp. 694-698.
ISSN 0268–2575
Abstract
BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym
435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a
five-level, three-variable, central composite rotatable design (CCRD) was employed to evaluate the interactive
effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 ◦C) and enzyme
amount (120–160 mg).
RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of
betulinic acid ester produced.
CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h,
temperature of 53.1 ◦C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum
conditions, which compared well with the maximum predicted value of 47.6%.
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