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Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system


Citation

Hadi, Sofijan and Tan, Joo Shun and Abbasiliasi, Sahar and Mustafa, Shuhaimi and Puspaningsih, Nyoman Tri and Kadkhodaei, Saeid and Ariff, Arbakariya (2017) Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system. Separation Science and Technology, 52 (5). 834 - 842. ISSN 0149-6395; ESSN: 1520-5754

Abstract

A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VR of 2.3, crude loading (CL) of 30% (w/w) at pH 6.


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Additional Metadata

Item Type: Article
Divisions: Halal Products Research Institute
Institute of Bioscience
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.1080/01496395.2016.1268159
Publisher: Taylor & Francis
Keywords: Aqueous two-phase system; β-xylosidase; Purification; Polyethylene glycol
Depositing User: Mas Norain Hashim
Date Deposited: 25 Nov 2022 01:39
Last Modified: 25 Nov 2022 01:39
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/01496395.2016.1268159
URI: http://psasir.upm.edu.my/id/eprint/62923
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