Citation
Hadi, Sofijan and Tan, Joo Shun and Abbasiliasi, Sahar and Mustafa, Shuhaimi and Puspaningsih, Nyoman Tri and Kadkhodaei, Saeid and Ariff, Arbakariya
(2017)
Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system.
Separation Science and Technology, 52 (5).
834 - 842.
ISSN 0149-6395; ESSN: 1520-5754
Abstract
A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VR of 2.3, crude loading (CL) of 30% (w/w) at pH 6.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Halal Products Research Institute Institute of Bioscience Institute of Tropical Agriculture |
| DOI Number: | https://doi.org/10.1080/01496395.2016.1268159 |
| Publisher: | Taylor & Francis |
| Keywords: | Aqueous two-phase system; β-xylosidase; Purification; Polyethylene glycol |
| Depositing User: | Mas Norain Hashim |
| Date Deposited: | 25 Nov 2022 01:39 |
| Last Modified: | 25 Nov 2022 01:39 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/01496395.2016.1268159 |
| URI: | http://psasir.upm.edu.my/id/eprint/62923 |
| Statistic Details: | View Download Statistic |
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