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Generation of transgenic rice expressing cyclotide precursor Oldenlandia affinis kalata B1 protein


Citation

Lim, Yi Yi and Lai, Kok Song (2017) Generation of transgenic rice expressing cyclotide precursor Oldenlandia affinis kalata B1 protein. The Journal of Animal and Plant Sciences, 27 (2). pp. 667-671. ISSN 1018-7081

Abstract

Golden apple snail (Pomacea canaliculata) is a devastating pest on rice that causes heavy economic losses in South East Asia. In this study, we transformed mature seed-derived rice callus with plasmid containing Oldenlandia affinis kalata B1 (Oak1) gene encoding precursor's protein for potent molluscicidal agent of cyclotidekalata B1 targeting the golden apple snails. A total of 11 independent T0 transformants were recovered and 7 were positive Oak1transformants according to genomic PCR analysis. The Oak1 mRNA transcript was successfully detected on all the tested T0 transformants using real-time PCR. Further immunoprecipitation experiment using specific Oak1 antibodies confirmed the presence of Oak1 protein expression in the transformants. We report, for the first time, the generation of transgenic rice plants expressing Oak1 as a potential crop protection strategy against the golden apple snail pest.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher: Pakistan Agricultural Scientists Forum
Keywords: Cyclotidekalata B1; Golden apple snail; Oak1; Oryzasativa ssp. Indica
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 07 Mar 2019 03:39
Last Modified: 07 Mar 2019 03:39
URI: http://psasir.upm.edu.my/id/eprint/61949
Statistic Details: View Download Statistic

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