UPM Institutional Repository

G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development


Citation

Ooi, Kah Kooi and Yeo, Chien Ing and Mahandaran, Theventhiran and Ang, Kok Pian and Md Akim, Abdah and Cheah, Yoke Kqueen and Seng, Hoi Ling and Tiekink, Edward R.T. (2017) G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development. Journal of Inorganic Biochemistry, 166. pp. 173-181. ISSN 0162-0134; ESSN: 1873-3344

Abstract

Phosphanegold(I) thiolates, Ph3PAu[SC(OR) = NPh], R = Me (1), Et (2) and iPr (3), were previously shown to be significantly cytotoxic toward HT-29 cancer cells and to induce cell death by both intrinsic and extrinsic apoptotic pathways whereby 1 activated the p73 gene, and each of 2 and 3 activated p53; 2 also caused apoptotic cell death via the c-Jun N-terminal kinase/mitogen-activated protein kinase pathway. Apoptosis pathways have been further evaluated by mitochondrial cytochrome c measurements and annexin V screening, confirming apoptotic pathways of cell death. Cell cycle analysis showed the majority of treated HT-29 cells were arrested at the G2/M checkpoint after 24 h; results of both assays were confirmed by changes in populations of relevant genes (PCR array analysis). Cell invasion studies showed inhibition of metastasis through Matrigel™ matrix to 17–22% cf. untreated cells. LC50 values were determined in zebrafish (8.36, 8.17, and 7.64 μM for 1–3). Finally, the zebrafish tolerated doses of 1 and 2 up to 0.625 μM, and 3 was tolerated at even higher doses of up to 1.25 μM.


Download File

[img]
Preview
Text
Cell cycle arrest on HT-29 cancer cells and toxicity assessment .pdf

Download (78kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.jinorgbio.2016.11.008
Publisher: Elsevier
Keywords: Gold(I) compounds; Carbonimidothioates; Phosphanegold(I) thiolates; Apoptosis; HT-29 cancer cell; Zebrafish
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 27 Feb 2019 08:48
Last Modified: 27 Feb 2019 08:48
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jinorgbio.2016.11.008
URI: http://psasir.upm.edu.my/id/eprint/61930
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item