Citation
Abstract
The aim of this study was to engineer skeletal muscle tissue for repair abdominal wall defects. Myoblast were seeded onto the scaffolds and cultivated in vitro for 5 days. Full thickness abdominal wall defects (3 × 4 cm) were created in 18 male New Zealand white rabbits and randomly divided into two equal groups. The defects of the first group were repaired with myoblast-seeded-bovine tunica vaginalis whereas the second group repaired with non-seeded-bovine tunica vaginalis and function as a control. Three animals were sacrificed at 7th, 14th, and 30th days of post-implantation from each group and the explanted specimens were subjected to macroscopic and microscopic analysis. In every case, seeded scaffolds have better deposition of newly formed collagen with neo-vascularisation than control group. Interestingly, multinucleated myotubes and myofibers were only detected in cell-seeded group. This study demonstrated that myoblast-seeded-bovine tunica vaginalis can be used as an effective scaffold to repair severe and large abdominal wall defects with regeneration of skeletal muscle tissue.
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Official URL or Download Paper: http://dx.doi.org/10.1007/s10856-010-4007-7
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Veterinary Medicine Faculty of Biotechnology and Biomolecular Sciences |
| DOI Number: | https://doi.org/10.1007/s10856-010-4007-7 |
| Publisher: | Springer |
| Keywords: | Skeletal muscle tissue; Abdominal wall defect; Myoblast-seeded-bovine tunica vaginalis |
| Depositing User: | Azizan Arshad |
| Date Deposited: | 12 May 2010 08:02 |
| Last Modified: | 16 Nov 2016 07:32 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10856-010-4007-7 |
| URI: | http://psasir.upm.edu.my/id/eprint/6175 |
| Statistic Details: | View Download Statistic |
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