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Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents


Citation

Ng, Lin Cieh and Sulaiman, Safwan and Mokhtar, Mohd Noriznan and Naim, Mohd Nazli (2017) Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents. Process Biochemistry (56). 81 - 89. ISSN 1359-5113; ESSN: 1873-3298

Abstract

Enzyme immobilization via covalent binding provides a strong interaction between enzyme and support material. In this study, the effect of different coupling agents (spacer arms and ligands) in cyclodextrin glucanotransferase (CGTase) immobilization on bleached kenaf microfiber as a support matrix was investigated. The immobilized CGTase properties such as storage stability, thermal stability and reusability were evaluated. Immobilized CGTases on microfiber resulted in 0.162–0.24 U/mg-fiber when 55.6 U/mL of CGTase activity was initially added during the immobilization. The highest storage stability (60 °C) was shown by CGTase that was immobilized with ethylenediamine and o-phthalaldehyde, whereby 60% of its activity remained after 15 days. Its high stability was also confirmed by the lowest deactivation constant, kd that was obtained at 25 °C (0.0161 day−1) and 60 °C (0.0361 day−1). The CGTase immobilized using ethylenediamine and glutaraldehyde has shown the best retention of enzyme activity up to 72.72% after 12 cycles of batch reaction. The results indicate that kenaf microfiber has potential to be applied as a support for enzyme immobilization and its enzymatic properties were affected by the coupling agents.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
DOI Number: https://doi.org/10.1016/j.procbio.2017.02.025
Publisher: Elsevier
Keywords: Cyclodextrin glucanotransferase (CGTase); Bleached kenaf microfiber; Covalent immobilization; Spacer arm; Ligand
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 20 Mar 2019 08:56
Last Modified: 20 Mar 2019 08:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.procbio.2017.02.025
URI: http://psasir.upm.edu.my/id/eprint/61016
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