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Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests


Citation

Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2015) Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests. Food Additives & Contaminants: Part A, 32 (7). pp. 1023-1028. ISSN 1944-0049; ESSN: 1944-0057

Abstract / Synopsis

Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Halal Products Research Institute
DOI Number: https://doi.org/10.1080/19440049.2015.1039605
Publisher: Taylor & Francis
Keywords: Edible bird's nest; Gelatine; Adulterant; ELISA
Depositing User: Nabilah Mustapa
Date Deposited: 07 Jun 2018 14:24
Last Modified: 07 Jun 2018 14:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/19440049.2015.1039605
URI: http://psasir.upm.edu.my/id/eprint/60503
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