Citation
Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir
(2015)
Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests.
Food Additives & Contaminants: Part A, 32 (7).
pp. 1023-1028.
ISSN 1944-0049; ESSN: 1944-0057
Abstract
Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.
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Official URL or Download Paper: https://www.tandfonline.com/doi/abs/10.1080/194400...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Medicine and Health Science Halal Products Research Institute |
| DOI Number: | https://doi.org/10.1080/19440049.2015.1039605 |
| Publisher: | Taylor & Francis |
| Keywords: | Edible bird's nest; Gelatine; Adulterant; ELISA |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 07 Jun 2018 06:24 |
| Last Modified: | 07 Jun 2018 06:24 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/19440049.2015.1039605 |
| URI: | http://psasir.upm.edu.my/id/eprint/60503 |
| Statistic Details: | View Download Statistic |
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