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Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat


Citation

Thung, Tze Young and Chin, Yih Zhet and Mohd Shahril, Najwa and Anyi, Ubong and New, Chia Yeung and Bahumaish, Ramzi Othman Saeed and Kuan, Chee Hao and Mahyudin, Nor Ainy and Basri, Dayang Fredalina and Wan Mohamed Radzi, Che Wan Jasimah and Radu, Son (2017) Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat. International Food Research Journal, 24 (4). pp. 1826-1830. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Salmonellosis is an important public health problem and causes large economic losses in the poultry industry. The emergence of molecular technology has opened various possibilities for constructing tailor-made proteins, particularly protein E from bacteriophage PhiX174 for the production of bacterial ghosts (BGs) applied in vaccines purposes. In the present study, the plamdaPRcI-Elysis plasmid carrying the PhiX174 lysis gene E and thermo-sensitive lamda PR-cl857 regulatory system was constructed. Two Salmonella Enteritidis (SE-2 and SE-4) and one Salmonella Typhimurium (ST-4) isolates were able to uptake the lysis plasmid via electrotransformation. Generation of ghosts was enhanced by increasing the incubation temperature up to 42 ̊C. Cell viability of SE-2, SE-4 and ST-4 decreased ranging in log 2.7 to log 4.1 cycles after lysis induction. Moreover, SE-2 and SE-4 exhibited the earliest reduction of CFU after 3 h of incubation. Our results may provide a promising avenue for the development of Salmonella BGs vaccines.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Institute of Tropical Agriculture and Food Security
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Salmonella; Bacterial ghost; Lysis gene E; Electrotransformation
Depositing User: Nabilah Mustapa
Date Deposited: 15 Jan 2018 02:42
Last Modified: 15 Jan 2018 02:42
URI: http://psasir.upm.edu.my/id/eprint/58375
Statistic Details: View Download Statistic

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