Citation
Abstract
Salmonellosis is an important public health problem and causes large economic losses in the poultry industry. The emergence of molecular technology has opened various possibilities for constructing tailor-made proteins, particularly protein E from bacteriophage PhiX174 for the production of bacterial ghosts (BGs) applied in vaccines purposes. In the present study, the plamdaPRcI-Elysis plasmid carrying the PhiX174 lysis gene E and thermo-sensitive lamda PR-cl857 regulatory system was constructed. Two Salmonella Enteritidis (SE-2 and SE-4) and one Salmonella Typhimurium (ST-4) isolates were able to uptake the lysis plasmid via electrotransformation. Generation of ghosts was enhanced by increasing the incubation temperature up to 42 ̊C. Cell viability of SE-2, SE-4 and ST-4 decreased ranging in log 2.7 to log 4.1 cycles after lysis induction. Moreover, SE-2 and SE-4 exhibited the earliest reduction of CFU after 3 h of incubation. Our results may provide a promising avenue for the development of Salmonella BGs vaccines.
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Official URL or Download Paper: http://www.ifrj.upm.edu.my/24%20(04)%202017/(62).p...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Food Science and Technology Institute of Tropical Agriculture and Food Security |
| Publisher: | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| Keywords: | Salmonella; Bacterial ghost; Lysis gene E; Electrotransformation |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 15 Jan 2018 02:42 |
| Last Modified: | 15 Jan 2018 02:42 |
| URI: | http://psasir.upm.edu.my/id/eprint/58375 |
| Statistic Details: | View Download Statistic |
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