Citation
Abstract
Previous studies carried out in mouse 3T3-L1 cell culture have shown that conjugated linoleic acid (CLA) inhibited adipocyte differentiation. The present study was undertaken to investigate the effect of cis-9, trans-11 and trans-10, cis-12 CLA isomers on morphological changes and on mRNA expressions in the in vitro adipocyte isolated from specific pathogen-free (SPF) chicken. The adipocytes were isolated from SPF chicken and cultured in differentiation-induction medium with different concentrations of cis-9, trans-11 and trans-10, cis-12 CLA. After day 7, the adipocyte differentiations were monitored morphologically and mRNA expressions of lipoprotein lipase (LPL) and acyl-coenzyme A binding domain containing 5 (ACBD 5) were quantified by real-time polymerase chain reaction (PCR) analysis. Our data suggested that cis-9, trans-11 CLA downregulated the expression of LPL and ACBD 5 genes, which was concurrently observed with decrease in the adipocyte area size and cell number compared to the control and trans-10, cis-12 treated groups. Based on this finding, we concluded that dietary CLA modulate fat reduction in chicken via alteration of transcription of key adipogenic genes and adipose cellularity.
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Official URL or Download Paper: http://www.tandfonline.com/doi/full/10.1080/182805...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Veterinary Medicine Institute of Bioscience |
| DOI Number: | https://doi.org/10.1080/1828051X.2016.1277961 |
| Publisher: | Taylor & Francis |
| Keywords: | Adipocytes; Chicken; Conjugated linoleic acid; Gene expression; Fatty acids |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 04 Jul 2017 02:46 |
| Last Modified: | 04 Jul 2017 02:46 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/1828051X.2016.1277961 |
| URI: | http://psasir.upm.edu.my/id/eprint/56206 |
| Statistic Details: | View Download Statistic |
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