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Distribution of bacteriophages in food and environment samples


Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari and Thung, Tze Young and New, Chia Yeung and Tang, John Yew Huat and Basri, Dayang Fredalina and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2017) Distribution of bacteriophages in food and environment samples. International Food Research Journal, 24 (2). pp. 888-896. ISSN 1985-4668; ESSN: 2231-7546


Foodborne pathogens have become a constant threat to the consumer and food industry. Reduce efficacy of antibiotics with emergence of resistant bacteria has limited the opportunities for controlling pathogenic bacteria in food commodities and treating foodborne infections. Bacteriophages can be a promising alternative for alleviate the risk of transmitting pathogenic bacteria via food commodities. Therefore, this research was conducted to find distribution of bacteriophages in diverse niches in order to identify suitable sources for isolating bacteriophages to use controlling foodborne pathogens. Firstly bacterial strains were screened for lysogenic and selected suitable host bacterial strains were used for isolating and determining bacteriophage titer in fresh raw food and environmental samples. Eighteen different lytic bacteriophages effective against Campylobacter, S. aureus, L. monocytogenes and E. coli were isolated from this study. Bacteriophages titer was determined within range of 102 to 1010 PFU/mL and bacteriophages were most frequently isolated from chicken (60%) samples. The isolated bacteriophages could be potential candidates for controlling foodborne diseases.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Institute of Tropical Agriculture
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Foodborne pathogens; Bacteriophages; Double layer assay; Plaques; Bacteriophage titer
Depositing User: Nabilah Mustapa
Date Deposited: 03 Jul 2017 09:40
Last Modified: 05 Jul 2017 03:26
URI: http://psasir.upm.edu.my/id/eprint/56146
Statistic Details: View Download Statistic

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