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Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology


Citation

Mat Radzi, Salina and Basri, Mahiran and Salleh, Abu Bakar and Ariff, Arbakariya and Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2006) Optimisation study of large-scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology. Journal of Chemical Technology and Biotechnology, 81 (3). pp. 374-380. ISSN 0268-2575; ESSN: 1097-4660

Abstract / Synopsis

An optimisation study of the large-scale enzymatic synthesis of a liquid wax ester from oleic acid and oleyl alcohol using Novozym 435 was carried out. Investigations were performed in batch mode with a stirred tank reactor (STR) with one multi-bladed impeller. Response surface methodology (RSM) based on a five-level, three-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of various parameters. The parameters are amount of enzyme (A) (90–120 g), impeller speed (B) (100–400 rpm) and temperature (C) (40–60 °C). The optimum conditions derived via RSM at a fixed reaction time of 1 h were successfully optimised as A = 104 g, B = 388.0 rpm and C = 49.7 °C. The actual experimental yield was 96.7% under the optimum conditions, which compared well with the maximum predicted value of 97.6%.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1002/jctb.1404
Publisher: Society of Chemical Industry
Keywords: Response surface methodology; Central composite rotatable design; Liquid wax ester; Novozym 435; Esterification
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Apr 2010 06:35
Last Modified: 28 Sep 2016 03:23
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jctb.1404
URI: http://psasir.upm.edu.my/id/eprint/5606
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