Citation
Abstract
An optimisation study of the large-scale enzymatic synthesis of a liquid wax ester from oleic acid and oleyl alcohol using Novozym 435 was carried out. Investigations were performed in batch mode with a stirred tank reactor (STR) with one multi-bladed impeller. Response surface methodology (RSM) based on a five-level, three-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of various parameters. The parameters are amount of enzyme (A) (90–120 g), impeller speed (B) (100–400 rpm) and temperature (C) (40–60 °C). The optimum conditions derived via RSM at a fixed reaction time of 1 h were successfully optimised as A = 104 g, B = 388.0 rpm and C = 49.7 °C. The actual experimental yield was 96.7% under the optimum conditions, which compared well with the maximum predicted value of 97.6%.
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Official URL or Download Paper: http://onlinelibrary.wiley.com/wol1/doi/10.1002/jc...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Science Faculty of Biotechnology and Biomolecular Sciences |
| DOI Number: | https://doi.org/10.1002/jctb.1404 |
| Publisher: | Society of Chemical Industry |
| Keywords: | Response surface methodology; Central composite rotatable design; Liquid wax ester; Novozym 435; Esterification |
| Depositing User: | Erni Suraya Abdul Aziz |
| Date Deposited: | 16 Apr 2010 06:35 |
| Last Modified: | 28 Sep 2016 03:23 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jctb.1404 |
| URI: | http://psasir.upm.edu.my/id/eprint/5606 |
| Statistic Details: | View Download Statistic |
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