Citation
Alavijeh, Pardis Karimi and Halimoon, Normala and Zakaria, Mohamad Pauzi and Wan Johari, Wan Lutfi and Karimi, Benyamin
(2013)
Crude oil biodegradation using isolated bacteria from oil polluted soil.
In: International Conference on Environmental Forensics 2013, 11-14 Nov. 2013, Marriot Hotel, Putrajaya, Malaysia. (pp. 127-129).
Abstract
Contamination due to the spillage of petroleum or petroleum-derived hydrocarbons on different ecosystems has become critical issues to environment and living things. The use of native bacteria as an environmental friendly treatment of hydrocarbons pollution is very promising. The objective of the study is to identify the native hydrocarbon-degraders isolated from oil refinery. Bacteria from crude oil were isolated and cultured in enrichment media. The isolated strains were then cultured in different oil concentration. The isolated bacterial strains were Gram-stained and further identified via 16S rRNA sequencing. The resultant sequences were then aligned with the available online database for identification of the bacteria. The isolated bacteria belonged to families of Pseudomonadaceae and Moraxellaceae (Acinetobacter genus). The bacteria show high degradation of hydrocarbon and can be used to remediate polluted soils in tropical environment.
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