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Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor


Citation

Shojaei, Taha Roodbar and Mohd Salleh, Mohamad Amran and Sijam, Kamaruzaman and Abdul Rahim, Raha and Mohsenifar, Afshin and Safarnejad, Reza and Tabatabaei, Meisam (2016) Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 169. pp. 216-222. ISSN 1386-1425; ESSN: 1873-3557

Abstract

Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat protein (CP) of CTV, and the CP were immobilized on the surface of gold nanoparticles (AuNPs) to develop a specific and sensitive fluorescence resonance energy transfer (FRET)-based nanobiosensor for detecting CTV. The maximum FRET efficiency for the developed nano-biosensor was observed at 60% in AuNPs-CP/QDs-Ab ratio of 1:8.5. The designed system showed higher sensitivity and specificity over enzyme linked immunosorbent assay (ELISA) with a limit of detection of 0.13μgmL(-1) and 93% and 94% sensitivity and specificity, respectively. As designed sensor is rapid, sensitive, specific and efficient in detecting CTV, this could be envisioned for diagnostic applications, surveillance and plant certification program.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Institute of Advanced Technology
DOI Number: https://doi.org/10.1016/j.saa.2016.06.052
Publisher: Elsevier
Keywords: Bioconjugation; Cadmium-telluride quantum dots; Fluorometric immunoassay; Gold nanoparticles; Nanobiosensor; Quenching
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 14 Sep 2017 09:52
Last Modified: 14 Sep 2017 09:52
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.saa.2016.06.052
URI: http://psasir.upm.edu.my/id/eprint/55460
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