Citation
Abstract
Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat protein (CP) of CTV, and the CP were immobilized on the surface of gold nanoparticles (AuNPs) to develop a specific and sensitive fluorescence resonance energy transfer (FRET)-based nanobiosensor for detecting CTV. The maximum FRET efficiency for the developed nano-biosensor was observed at 60% in AuNPs-CP/QDs-Ab ratio of 1:8.5. The designed system showed higher sensitivity and specificity over enzyme linked immunosorbent assay (ELISA) with a limit of detection of 0.13μgmL(-1) and 93% and 94% sensitivity and specificity, respectively. As designed sensor is rapid, sensitive, specific and efficient in detecting CTV, this could be envisioned for diagnostic applications, surveillance and plant certification program.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture Faculty of Biotechnology and Biomolecular Sciences Faculty of Engineering Institute of Advanced Technology |
| DOI Number: | https://doi.org/10.1016/j.saa.2016.06.052 |
| Publisher: | Elsevier |
| Keywords: | Bioconjugation; Cadmium-telluride quantum dots; Fluorometric immunoassay; Gold nanoparticles; Nanobiosensor; Quenching |
| Depositing User: | Mohd Hafiz Che Mahasan |
| Date Deposited: | 14 Sep 2017 09:52 |
| Last Modified: | 14 Sep 2017 09:52 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.saa.2016.06.052 |
| URI: | http://psasir.upm.edu.my/id/eprint/55460 |
| Statistic Details: | View Download Statistic |
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