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Characterization of novel type I ribosome-inactivating proteins isolated from oil palm (Elaeis guineensis) inoculated with Ganoderma boninense, the causal agent of basal stem rot


Citation

Sargolzaei, M. and Ho, C. L. and Wong, M. Y. (2016) Characterization of novel type I ribosome-inactivating proteins isolated from oil palm (Elaeis guineensis) inoculated with Ganoderma boninense, the causal agent of basal stem rot. Physiological and Molecular Plant Pathology, 94. pp. 53-61. ISSN 0885-5765; ESSN: 1096-1178

Abstract / Synopsis

Type I RIPs have not been identified in oil palm. EgRIP-1a and EgRIP-1b transcripts were partially isolated from roots and basal stems of oil palm seedlings with high similarity to type I RIPs. Expression levels were altered during plant-pathogen interaction and their proteins were 28–30 kD with an estimated pI value of 10.0. Depurination of yeast 26S rRNA by EgRIP proteins demonstrated specific ribosome-inactivating activity. EgRIP proteins showed inhibition on G. boninense mycelial growth with 44.1% at 5 DAI. It was concluded that the novel EgRIPs are type I and demonstrated to have antifungal effect against G. boninense.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.1016/j.pmpp.2016.04.002
Publisher: Academic Press
Keywords: Cation-exchange column; N-glycosidase activity; Saccharomyces cerevisiae; rRNA depurination; Fungal inhibition
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 15 Aug 2018 09:57
Last Modified: 15 Aug 2018 09:57
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.pmpp.2016.04.002
URI: http://psasir.upm.edu.my/id/eprint/55269
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