Citation
Sargolzaei, Maryam and Ho, Chai Ling and Wong, Mui Yun
(2016)
Characterization of novel type I ribosome-inactivating proteins isolated from oil palm (Elaeis guineensis) inoculated with Ganoderma boninense, the causal agent of basal stem rot.
Physiological and Molecular Plant Pathology, 94.
pp. 53-61.
ISSN 0885-5765; ESSN: 1096-1178
Abstract
Type I RIPs have not been identified in oil palm. EgRIP-1a and EgRIP-1b transcripts were partially isolated from roots and basal stems of oil palm seedlings with high similarity to type I RIPs. Expression levels were altered during plant-pathogen interaction and their proteins were 28–30 kD with an estimated pI value of 10.0. Depurination of yeast 26S rRNA by EgRIP proteins demonstrated specific ribosome-inactivating activity. EgRIP proteins showed inhibition on G. boninense mycelial growth with 44.1% at 5 DAI. It was concluded that the novel EgRIPs are type I and demonstrated to have antifungal effect against G. boninense.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture Faculty of Biotechnology and Biomolecular Sciences Institute of Tropical Agriculture |
| DOI Number: | https://doi.org/10.1016/j.pmpp.2016.04.002 |
| Publisher: | Elsevier |
| Keywords: | Cation-exchange column; N-glycosidase activity; Saccharomyces cerevisiae; rRNA depurination; Fungal inhibition |
| Depositing User: | Mohd Hafiz Che Mahasan |
| Date Deposited: | 15 Aug 2018 01:57 |
| Last Modified: | 23 Oct 2019 00:53 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.pmpp.2016.04.002 |
| URI: | http://psasir.upm.edu.my/id/eprint/55269 |
| Statistic Details: | View Download Statistic |
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