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Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines


Citation

Santhanam, Ramesh Kumar and Ahmad, Syahida and Abas, Faridah and Ismail, Intan Safinar and Rukayadi, Yaya and Akhtar, Muhammad Tayyab and Shaari, Khozirah (2016) Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines. Molecules, 21 (652). pp. 1-13. ISSN 1431-5157; ESSN: 1420-3049

Abstract

Zanthoxylum rhetsa is an aromatic tree, known vernacularly as "Indian Prickly Ash". It has been predominantly used by Indian tribes for the treatment of many infirmities like diabetes, inflammation, rheumatism, toothache and diarrhea. In this study, we identified major volatile constituents present in different solvent fractions of Z. rhetsa bark using GC-MS analysis and isolated two tetrahydrofuran lignans (yangambin and kobusin), a berberine alkaloid (columbamine) and a triterpenoid (lupeol) from the bioactive chloroform fraction. The solvent fractions and purified compounds were tested for their cytotoxic potential against human dermal fibroblasts (HDF) and mouse melanoma (B16-F10) cells, using the MTT assay. All the solvent fractions and purified compounds were found to be non-cytotoxic to HDF cells. However, the chloroform fraction and kobusin exhibited cytotoxic effect against B16-F10 melanoma cells. The presence of bioactive lignans and alkaloids were suggested to be responsible for the cytotoxic property of Z. rhetsa bark against B16-F10 cells.


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Official URL or Download Paper: http://www.mdpi.com/journal/molecules

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.3390/molecules21060652
Publisher: MDPI
Keywords: GC-MS; NMR spectroscopy; Zanthoxylum rhetsa; Alkaloids; Cytotoxicity; Lignans; Triterpenes
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 22 Jan 2018 10:07
Last Modified: 22 Jan 2018 10:07
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules21060652
URI: http://psasir.upm.edu.my/id/eprint/55201
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