Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system

Citation

Amin Yusof, Nurul Atikah and Mat Hadzir, Nursyamsyila and Ashari, Siti Efliza (2016) Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system. Journal of Applied Chemistry and Biotechnology, 2016. art. no. 5149326. pp. 1-5. ISSN 2356-7171; ESSN: 2314-6923

Abstract

Betulinic acid amide was synthesized from the enzymatic reaction of betulinic acid and butylamine catalysed by Novozym 435. The effects of different reaction parameters, such as effect of reaction time, reaction temperature, amount of enzyme, and substrate molar ratio (betulinic acid : butylamine), were studied and conventionally optimised. Based on this study, the enzymatic synthesis of betulinic acid amide was found to be 64.6% at the optimum conditions of 24 h, 40°C, 100 mg enzyme, and 1 : 1 substrate molar ratio in 9 : 1 mixture of chloroform and hexane as solvent. The identification of final product was carried out using TLC, melting point, and FTIR and NMR showed the presence of betulinic acid amide.

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Additional Metadata

Item Type:	Article
Subject:	Lipase- catalysed synthesis; Betulinic acid amide; Solvent system
Divisions:	Faculty of Science
DOI Number:	https://doi.org/10.1155/2016/5149326
Publisher:	Hindawi Publishing Corporation
Depositing User:	Nurul Ainie Mokhtar
Date Deposited:	20 Mar 2018 03:45
Last Modified:	20 Mar 2018 03:45
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2016/5149326
URI:	http://psasir.upm.edu.my/id/eprint/54479
Statistic Details:	View Download Statistic

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