Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots

Citation

Shojaei, Taha Roodbar and Mohd Salleh, Mohamad Amran and Sijam, Kamaruzaman and Abdul Rahim, Raha and Mohsenifar, Afshin and Safarnejad, Reza and Tabatabaei, Meisam (2016) Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots. Microchimica Acta, 183 (7/July). pp. 2277-2287. ISSN 0026-3672; ESSN:1436-5073

Abstract

Cadmium-telluride quantum dots (QDs) were conjugated to an antibody (Ab) against Citrus tristeza virus (CTV), while the coat protein (CP) of the CTV was immobilized on the surface of carbon nanoparticles (CNPs). Following immunobinding of the QD-Ab and the CP-loaded CNPs, the fluorescence of the CdTe QDs was quenched by the CNPs. This effect was exploited to design a detection assay for the CTV which was found more sensitive and specific than the existing enzyme linked immunosorbent assay (ELISA). The limit of detection was measured at about 220 ng⋅ mL‾1 of CTV. The Stern-Volmer plot of the CNPs-QD quencher pair showed a positive deviation from linearity which was ascribed to the presence of both static and dynamic quenching.

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Additional Metadata

Item Type:	Article
Subject:	Bioassay; Competitive quenching; Stern-Volmer relationship; ELISA; TEM; FTIR; CdTe; Synthesis; Bioconjugation
Divisions:	Faculty of Agriculture Faculty of Biotechnology and Biomolecular Sciences Faculty of Engineering Institute of Advanced Technology
DOI Number:	https://doi.org/10.1007/s00604-016-1867-7
Publisher:	Springer Verlag
Depositing User:	Nurul Ainie Mokhtar
Date Deposited:	14 Mar 2018 01:11
Last Modified:	14 Mar 2018 01:11
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s00604-016-1867-7
URI:	http://psasir.upm.edu.my/id/eprint/54334
Statistic Details:	View Download Statistic

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