Citation
Abstract
The potential use of fed-batch cultivation (FBC) for improvement of the production of Lactobacillus salivarius I 24 biomass for subsequent use as probiotics was studied using a 2-L stirredtank bioreactor. Three different constant feeding rates (0.1, 0.05, and 0.033 L/h) were applied in FBCs and their effect on carbon metabolism was evaluated. The carbon flux for cell built-up with reduction in lactic acid synthesis was observed in the fed-batch as compared to the batch cultivation mode. The viable cell number obtained in the constant FBC (CFBC) operated at a feeding rate of 0.05 L/h was 8 times higher (10.7×1010 CFU/mL) than that recorded in the batch cultivation. This gave the viable cell yield based on glucose consumed for CFBC of 26 times higher (11.3×1012 CFU/gGlucose) than the batch cultivation. This study demonstrated CFBC, which is simple with minimal use of process control equipment, has an industrial potential for improvement of probiotic production.
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Official URL or Download Paper: https://link.springer.com/article/10.1007/s10068-0...
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Additional Metadata
Item Type: | Article |
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Subject: | Fed-batch cultivation; Lactobacillus salivarius; Lactic acid bacteria; Probiotics; Carbon metabolism |
Divisions: | Faculty of Biotechnology and Biomolecular Sciences Institute of Bioscience |
DOI Number: | https://doi.org/10.1007/s10068-016-0217-1 |
Publisher: | Korean Society of Food Science and Technology |
Depositing User: | Nurul Ainie Mokhtar |
Date Deposited: | 20 Feb 2018 09:22 |
Last Modified: | 20 Feb 2018 09:22 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10068-016-0217-1 |
URI: | http://psasir.upm.edu.my/id/eprint/53897 |
Statistic Details: | View Download Statistic |
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