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Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar


Citation

Wong, Clement Kiing Fook and Lai, Kok Song and Wong, Mui Yun and Mahmood, Maziah (2016) Minimal inhibitory concentration of common selectable agents on recalcitrant Malaysian rice cultivar. Asian Journal of Agricultural Research, 10 (2). pp. 126-131. ISSN 1819-1894

Abstract

Determining the suitable and optimized Minimal Inhibitory Concentration (MIC) of the selectable agents is critical to successfully recover stably transformed plants. In this study, the MIC of five different antibiotics and one herbicide was determined for their inhibitory effect on the growth of a recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue. Antibiotics such as kanamycin, paromomycin and neomycin did not inhibit the shoot apices growth while geneticin (G-418) and hygromycin showed complete growth inhibition at 400 and 25 mg LG1 , respectively. Similarly, the herbicide basta indicated that 9 mg LG1 was sufficient to cause growth inhibition. To conclude, hygromycin and basta were suggested to the suitable selectable agent to select transformed rice shoot apices.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.3923/ajar.2016.126.131
Publisher: Science Alert
Keywords: Antibiotic; Herbicide; MR219; Shoot apices; Growth inhibition
Depositing User: Ms. Ainur Aqidah Hamzah
Date Deposited: 18 Mar 2022 02:00
Last Modified: 18 Mar 2022 02:00
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ajar.2016.126.131
URI: http://psasir.upm.edu.my/id/eprint/53288
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