Citation
Wong, Mui Yun and Huang, Jeng Sheng and Davis, Eric L.
(2006)
Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.).
Journal of Bioscience, 17 (1).
pp. 77-87.
ISSN 1985-3718; ESSN: 2180-4249
Abstract
Nitric oxide (NO) functions as a signal molecule in different biological processes in plants, including disease resistance. Its production is related to nitric oxide synthase (NOS). The application of chemicals that induce systemic resistance in plants did not induce NOS activity in pea, suggesting that NO functions upstream of salicylic acid (SA) in the signaling pathway of defense responses in plants. NOS activity was induced in both the incompatible and compatible interactions of pea with Ralstonia solanacearum and Pseudomonas syringae pv pisi, respectively, between 3 h to 6 h post-infiltration, indicating that NOS was involved in both resistance and disease development responses in pea. Antibodies raised against mammalian NOS did not have specificity in detecting a NOS-like protein in pea, suggesting that the pea NOS-like protein could be structurally different from mammalian NOS, and immunodetection of a plant NOS-like protein must be conducted with caution and verified with functional assays.
Download File
![[img]](http://psasir.upm.edu.my/51942/1.hassmallThumbnailVersion/Physiological%20and%20immunoblot%20analyses%20of%20a%20nitric%20oxide%20synthase%20%28NOS%29-like%20protein%20of%20pea%20%28Pisum%20sativum%20L.%29.pdf) ![](http://psasir.upm.edu.my/51942/1.haspreviewThumbnailVersion/Physiological%20and%20immunoblot%20analyses%20of%20a%20nitric%20oxide%20synthase%20%28NOS%29-like%20protein%20of%20pea%20%28Pisum%20sativum%20L.%29.pdf) Preview |
|
PDF (Abstract)
Physiological and immunoblot analyses of a nitric oxide synthase (NOS)-like protein of pea (Pisum sativum L.).pdf
Download (38kB)
| Preview
|
|
Additional Metadata
Item Type: |
Article
|
Divisions: |
Faculty of Agriculture |
Publisher: |
Penerbit Universiti Sains Malaysia |
Notes: |
Journal title changed to 'Tropical Life Sciences Research' since 2009 |
Keywords: |
Pea; Nitric oxide synthase (NOS); Ralstonia solanacearum; Pseudomonas syringae pv pisi |
Depositing User: |
Nabilah Mustapa
|
Date Deposited: |
03 May 2017 09:22 |
Last Modified: |
03 May 2017 09:22 |
URI: |
http://psasir.upm.edu.my/id/eprint/51942 |
Statistic Details: |
View Download Statistic |
Actions (login required)
![View Item View Item](/style/images/action_view.png) |
View Item |