Citation
Thung, Tze Young and Mohd Fadzil, Siti Norshafawatie and Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari and Chang, Wei San and Loo, Yuet Ying and Kuan, Chee Hao and New, Chia Yeung and Anyi, Ubong and Bahumaish, Ramzi Othman Saeed and Mahyudin, Nor Ainy and Basri, Dayang Fredalina and Wan Mohamed Radzi, Che Wan Jasimah and Radu, Son
(2017)
Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage.
International Food Research Journal, 24 (1).
pp. 450-454.
ISSN 1985-4668; ESSN: 2231-7546
Abstract
Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and shrimp) and sewage samples using 6 reference pathogen strains (Salmonella Enteritidis, Salmonella Typhimurium, Campylobacter jejuni, Vibrio parahaemolyticus, Listeria monocytogenes and Escherichia coli). A total of 29 bacteriophage isolates were obtained and further examined for titer via agar overlay assay. The titers were determined within the range of 108 to 1011 PFU/mL. Our results showed that diverse of bacteriophages are naturally present in a variety of foods.
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