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Production of urokinase by HT 1080 human kidney cell line


Citation

Roychoudhury, Pradip A. and Gomes, James and Bhattacharyay, Sanjoy K. and Abdulah, Norhafizah (1999) Production of urokinase by HT 1080 human kidney cell line. Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 27 (5&6). pp. 399-402. ISSN 1073-1199

Abstract / Synopsis

Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and urokinase production were observed to be the function of serum concentration in the medium. The maximum urokinase activity of 3.1 x 10-4 unit ml-1 was achieved in the bioreactor at around 65 h of batch culture. Since HT 1080 is an anchorage dependent cell line, therefore, the hydrodynamic effects on the cell line were investigated.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
DOI Number: https://doi.org/10.3109/10731199909117710
Publisher: Marcel Dekker
Keywords: Bioreactor; HT 1080 human kidney cell line; Hydrodynamic effects; Urokinase
Depositing User: Nabilah Mustapa
Date Deposited: 27 Apr 2017 18:09
Last Modified: 27 Apr 2017 18:09
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3109/10731199909117710
URI: http://psasir.upm.edu.my/id/eprint/50986
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