Citation
Roychoudhury, Pradip A. and Gomes, James and Bhattacharyay, Sanjoy K. and Abdulah, Norhafizah
(1999)
Production of urokinase by HT 1080 human kidney cell line.
Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 27 (5&6).
pp. 399-402.
ISSN 1073-1199
Abstract
Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and urokinase production were observed to be the function of serum concentration in the medium. The maximum urokinase activity of 3.1 x 10-4 unit ml-1 was achieved in the bioreactor at around 65 h of batch culture. Since HT 1080 is an anchorage dependent cell line, therefore, the hydrodynamic effects on the cell line were investigated.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Engineering |
| DOI Number: | https://doi.org/10.3109/10731199909117710 |
| Publisher: | Marcel Dekker |
| Keywords: | Bioreactor; HT 1080 human kidney cell line; Hydrodynamic effects; Urokinase |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 27 Apr 2017 10:09 |
| Last Modified: | 08 Aug 2024 00:44 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3109/10731199909117710 |
| URI: | http://psasir.upm.edu.my/id/eprint/50986 |
| Statistic Details: | View Download Statistic |
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