UPM Institutional Repository

Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species


Citation

Chan, Soon Choy and Tan, Soon Guan and Siraj, Siti Shapor and Mohd Yusoff, Khatijah (2005) Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species. Asian-Australasian Journal of Animal Sciences, 18 (11). pp. 1513-1518. ISSN 1011-2367; ESSN: 1976-5517

Abstract

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.


Download File

[img]
Preview
PDF (Abstract)
Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species.pdf

Download (35kB) | Preview
Official URL or Download Paper: http://www.ajas.info/journal/view.php?number=21149

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: https://doi.org/10.5713/ajas.2005.1513
Publisher: Asian-Australasian Association of Animal Production Societies
Keywords: Microsatellites; Cross-species amplification; Mystus nemurus; Pangasius micronemus; Clarias batrachus
Depositing User: Nabilah Mustapa
Date Deposited: 01 Feb 2017 04:37
Last Modified: 01 Feb 2017 04:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5713/ajas.2005.1513
URI: http://psasir.upm.edu.my/id/eprint/50079
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item