Citation
Abstract
The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.
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Official URL or Download Paper: http://www.ajas.info/journal/view.php?number=21149
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Science |
| DOI Number: | https://doi.org/10.5713/ajas.2005.1513 |
| Publisher: | Asian-Australasian Association of Animal Production Societies |
| Keywords: | Microsatellites; Cross-species amplification; Mystus nemurus; Pangasius micronemus; Clarias batrachus |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 01 Feb 2017 04:37 |
| Last Modified: | 01 Feb 2017 04:37 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5713/ajas.2005.1513 |
| URI: | http://psasir.upm.edu.my/id/eprint/50079 |
| Statistic Details: | View Download Statistic |
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