Citation
Abd Mutalib, Sahilah and Radu, Son and Lum, Keng Yeang and Lihan, Samuel
(2003)
Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE).
Journal of Tropical Agriculture and Food Science, 31 (1).
pp. 35-42.
ISSN 1394-9829; ESSN: 2289-9650
Abstract
Nine genomic DNA of Salmonella agona isolated from poultry sources were characterised by three variations of PCR-based techniques [polymerase chain reaction (PCR)-ribotyping, enterobacterial repetitive intergenic consensuspolymerase chain reaction (ERIC-PCR), and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)] and pulsed field gel electrophoresis (PFGE). These isolates originated from three different states of Peninsular Malaysia (Pulau Pinang, Negeri Sembilan and Selangor). The results of PCR-ribotyping, ERIC-PCR, RADP-PCR and PFGE to differentiate between nine isolates of S. agona were compared. The isolates were separated into three different genome types by PCR-ribotyping, nine and eight genome types by ERIC-PCR and PFGE, respectively.
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Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE).pdf
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Additional Metadata
| Item Type: |
Article
|
| Divisions: |
Faculty of Food Science and Biotechnology |
| Publisher: |
Malaysian Agricultural Research and Development Institute (MARDI) |
| Keywords: |
Salmonella agona; PCR-ribotyping; Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR); Random amplified polymorphic DNA-PCR (RAPD-PCR); Pulsed field gel electrophoresis (PFGE) |
| Depositing User: |
Nabilah Mustapa
|
| Date Deposited: |
29 Dec 2016 09:48 |
| Last Modified: |
29 Dec 2016 09:48 |
| URI: |
http://psasir.upm.edu.my/id/eprint/48891 |
| Statistic Details: |
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