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Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE)


Citation

Abd Mutalib, Sahilah and Radu, Son and Lum, Keng Yeang and Lihan, Samuel (2003) Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE). Journal of Tropical Agriculture and Food Science, 31 (1). pp. 35-42. ISSN 1394-9829; ESSN: 2289-9650

Abstract

Nine genomic DNA of Salmonella agona isolated from poultry sources were characterised by three variations of PCR-based techniques [polymerase chain reaction (PCR)-ribotyping, enterobacterial repetitive intergenic consensuspolymerase chain reaction (ERIC-PCR), and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)] and pulsed field gel electrophoresis (PFGE). These isolates originated from three different states of Peninsular Malaysia (Pulau Pinang, Negeri Sembilan and Selangor). The results of PCR-ribotyping, ERIC-PCR, RADP-PCR and PFGE to differentiate between nine isolates of S. agona were compared. The isolates were separated into three different genome types by PCR-ribotyping, nine and eight genome types by ERIC-PCR and PFGE, respectively.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
Publisher: Malaysian Agricultural Research and Development Institute (MARDI)
Keywords: Salmonella agona; PCR-ribotyping; Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR); Random amplified polymorphic DNA-PCR (RAPD-PCR); Pulsed field gel electrophoresis (PFGE)
Depositing User: Nabilah Mustapa
Date Deposited: 29 Dec 2016 09:48
Last Modified: 29 Dec 2016 09:48
URI: http://psasir.upm.edu.my/id/eprint/48891
Statistic Details: View Download Statistic

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