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Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen


Citation

Kimura, Yoshinobu and Yoshiie, Takeo and Kit, Woo Kwan and Maeda, Megumi and Kimura, Mariko and Tan, Siang Hee (2003) Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen. Bioscience, Biotechnology, and Biochemistry, 67 (10). pp. 2232-2239. ISSN 0916-8451; ESSN: 1347-6947

Abstract

The pollen of oil palm (Elaeis guineensis Jacq.) is a strong allergen and causes severe pollinosis in Malaysia and Singapore. In the previous study (Biosci. Biotechnol. Biochem., 64, 820-827 (2002)), from the oil palm pollens, we purified an antigenic glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients. In this report, we describe the structural analysis of sugar chains linked to palm pollen glycoproteins to confirm the ubiquitous occurrence of antigenic N-glycans in the allergenic pollen. N-Glycans liberated from the pollen glycoprotein mixture by hydrazinolysis were labeled with 2-aminopyridine followed by purification with a combination of size-fractionation HPLC and reversed-phase HPLC. The structures of the PA-sugar chains were analyzed by a combination of two-dimensional sugar chain mapping, electrospray ionization mass spectrometry (ESI-MS), and tandem MS analysis, as well as exoglycosidase digestions. The antigenic N-glycan bearing α1-3 fucose and/or β1-2 xylose residues accounts for 36.9% of total N-glycans: GlcNAc2Man3Xyl1Fuc1GlcNAc2 (24.6%), GlcNAc2Man3Xyl1GlcNAc2 (4.4%), Man3Xyl1Fuc1-GlcNAc2 (1.1%), GlcNAc1Man3Xyl1Fuc1GlcNAc2 (5.6%), and GlcNAc1Man3Xyl1GlcNAc2 (1.2%). The remaining 63.1% of the total N-glycans belong to the high-mannose type structure: Man9GlcNAc2 (5.8%), Man8GlcNAc2 (32.1%), Man7GlcNAc2 (19.9%), Man6GlcNAc2 (5.3%).


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Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.1271/bbb.67.2232
Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Keywords: Plant glycoprotein; Antigenic N-glycan; Pollen allergy; Oil palm pollen; Elaeis guineensis Jacq
Depositing User: Nabilah Mustapa
Date Deposited: 10 Oct 2016 07:49
Last Modified: 08 Aug 2024 00:35
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1271/bbb.67.2232
URI: http://psasir.upm.edu.my/id/eprint/48669
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