Simple Search:

Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli


Citation

Tan, Yung Chie and Ho, Wan Yong and Mohammed Alitheen, Noorjahan Banu and Wong, Mui Yun and Ho, Chai Ling (2016) Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli. International Journal of Peptide Research and Therapeutics, 22 (1). pp. 37-44. ISSN 1573-3149; ESSN: 1573-3904

Abstract / Synopsis

EgT2RIP is a type 2 ribosome-inactivating protein isolated from oil palm (Elaeis guineensis Jacq.). Its transcript abundance was reported to be up-regulated in oil palm roots upon inoculation of pathogenic fungus Ganoderma boninense in a recent study. This study aims to produce an active recombinant EgT2RIP protein for biological studies. The DNA fragments encoding Chain A (CA) and Chain B (CB) of EgT2RIP were cloned individually in an expression vector. Soluble CA and partially soluble CB were expressed in Escherichia coli Rosetta-gami 2 (DE3). Purified recombinant CA and CB were associated in a cysteine/cystine reduced/oxidized system, yielding a heterodimer protein (AB). The AB protein showed growth inhibitory activity against breast cancer cell lines (MCF-7) as well as non-tumorigenic breast epithelial cell line (MCF-10A) at IC50 = 1.4 and 10.9 μg mL−1, respectively. The active protein produced from this study may have the potential to be used for treatment in medical and agricultural fields.


Download File

[img]
Preview
PDF (Abstract)
Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli.pdf

Download (51kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.1007/s10989-015-9485-5
Publisher: Springer
Keywords: Elaeis guineensis; Ganoderma; N-glycosidase; Oil palm; Recombinant protein; Type 2 ribosome inactivating protein
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 20 May 2016 09:17
Last Modified: 20 May 2016 09:17
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10989-015-9485-5
URI: http://psasir.upm.edu.my/id/eprint/47431
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item