Citation
Abstract
EgT2RIP is a type 2 ribosome-inactivating protein isolated from oil palm (Elaeis guineensis Jacq.). Its transcript abundance was reported to be up-regulated in oil palm roots upon inoculation of pathogenic fungus Ganoderma boninense in a recent study. This study aims to produce an active recombinant EgT2RIP protein for biological studies. The DNA fragments encoding Chain A (CA) and Chain B (CB) of EgT2RIP were cloned individually in an expression vector. Soluble CA and partially soluble CB were expressed in Escherichia coli Rosetta-gami 2 (DE3). Purified recombinant CA and CB were associated in a cysteine/cystine reduced/oxidized system, yielding a heterodimer protein (AB). The AB protein showed growth inhibitory activity against breast cancer cell lines (MCF-7) as well as non-tumorigenic breast epithelial cell line (MCF-10A) at IC50 = 1.4 and 10.9 μg mL−1, respectively. The active protein produced from this study may have the potential to be used for treatment in medical and agricultural fields.
Download File
Official URL or Download Paper: http://link.springer.com/article/10.1007%2Fs10989-...
|
Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture Faculty of Biotechnology and Biomolecular Sciences Institute of Tropical Agriculture |
| DOI Number: | https://doi.org/10.1007/s10989-015-9485-5 |
| Publisher: | Springer |
| Keywords: | Elaeis guineensis; Ganoderma; N-glycosidase; Oil palm; Recombinant protein; Type 2 ribosome inactivating protein |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 20 May 2016 01:17 |
| Last Modified: | 20 May 2016 01:17 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10989-015-9485-5 |
| URI: | http://psasir.upm.edu.my/id/eprint/47431 |
| Statistic Details: | View Download Statistic |
Actions (login required)
 |
View Item |