Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules

Citation

Mustapha Kamil, Y. and Abu Bakar, Muhammad Hafiz and Mustapa, M. A. and Yaacob, Mohd. Hanif and Amir Hamzah, Amir Syahir and Mahdi, Mohd Adzir (2015) Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules. IEEE Photonics Journal, 7 (6). art. no. 3700109. pp. 1-9. ISSN 1943-0655

Abstract

We examine and demonstrate a biosensor using single-mode tapered fiber that has been immobilized with biorecognition molecules to sense targeted proteins. Interaction of evanescent waves with the external medium surrounding the tapered region produces an interferometric-patterned spectrum, which shifts correspondingly to any changes of refractive index (RI) in the external medium. The proposed setup managed to obtain an RI sensitivity and concentration sensitivity of 2526.8 nm/RIU and 20.368 nm/μM, respectively, which, to our knowledge, is highly sensitive when compared with previous studies. The dynamic performance, good specificity, and high sensitivity of the proposed method highlight an immensely beneficial choice for immunological diagnostics.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Biotechnology and Biomolecular Sciences Faculty of Engineering
DOI Number:	https://doi.org/10.1109/JPHOT.2015.2490486
Publisher:	Institute of Electrical and Electronics Engineers
Keywords:	Tapered microfiber; Refractive-index sensor; Biosensors; Biophotonics instrumentation
Depositing User:	Ms. Nida Hidayati Ghazali
Date Deposited:	02 May 2018 05:09
Last Modified:	02 May 2018 05:09
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1109/JPHOT.2015.2490486
URI:	http://psasir.upm.edu.my/id/eprint/46263
Statistic Details:	View Download Statistic

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