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Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia


Citation

Watanabe, Malaika and Nakao, R. and Babjee, Shaik Mohamed Amin and Moktar, Maizatul Akmal and Youn, J. H. and Qiu, Y. and Sugimoto, C. (2015) Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia. Tropical Biomedicine, 32 (2). pp. 390-398. ISSN 0127-5720

Abstract

A total of 44 Rhipicephalus sanguineus ticks collected from 23 dogs from Malaysia were screened for Rickettsia, Anaplasmataceae and Coxiella burnetii. Coxiella burnetii was detected in 59% (26/44) of ticks however Rickettsia and Anaplasmataceae were not detected in any of the ticks. In order to genotype the strains of C. burnetii, multispacer sequence typing (MST) was carried out using three different spacers. One of the spacers; Cox2 successfully amplified a fragment for which the full length sequence of 397 bp was obtained. The sequenced product revealed only a single nucleotide difference with the Cox2.3 type sequence.


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Official URL or Download Paper: https://pubmed.ncbi.nlm.nih.gov/26691268/

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Malaysian Society of Parasitology and Tropical Medicine
Keywords: Rhipicephalus; Rickettsia; Anaplasmataceae; Nucleotide
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 15 Apr 2021 02:57
Last Modified: 15 Apr 2021 02:57
URI: http://psasir.upm.edu.my/id/eprint/45531
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