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Association of TNF-α G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus


Citation

Ghodsian, N. and Akhlaghi, M. and Ramachandran, V. and Heidari, F. and Haghvirdizadeh, P. and Eshkoor, S. A. and Etemad, A. and Jamaluddin, J. A. and Ismail, Patimah (2015) Association of TNF-α G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus. Genetics and Molecular Research, 14 (4). pp. 18974-18979. ISSN 1676-5680

Abstract

This study aims to investigate the effects of tumor necrosis factor alpha (TNF-α) G308A gene polymorphism on essential hypertension (EHT) with or without type 2 diabetes mellitus (T2DM). The project was conducted on buccal epithelial and blood cells for case and control patients, respectively. Epithelial cells were obtained from the inner part of the cheeks. Techniques including DNA extraction, polymerase chain reaction (PCR), and restriction fragment length polymorphism (RFLP) were utilized to assess biomarkers of DNA damage. Our results demonstrated significant differences between wild and mutated genotypes among EHT patients without T2DM. We also found a significant association between wild and mutated allele frequencies in EHT patients (P < 0.05). Clinical characteristics between the groups (EHT with or without T2DM and controls) showed statistically significant association (P < 0.05). Overall, we show that G308A polymorphism of the TNF-αgene may be a significant genetic risk factor for EHT without T2DM patients in Malaysia.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.4238/2015.December.29.4
Publisher: Fundacao de Pesquisas Cientificas de Ribeirao Preto
Keywords: Association; TNF-α; G308A; Essential hypertension; Polymerase chain reaction; Restriction fragment length polymorphism
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 27 Oct 2016 01:47
Last Modified: 27 Oct 2016 01:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4238/2015.December.29.4
URI: http://psasir.upm.edu.my/id/eprint/43884
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