Citation
Abstract
BACKGROUND: Blast caused by the fungus Magnaporthe oryzae is a significant disease threat to rice across the world and is especially prevalent in Malaysia. An elite, early-maturing, high-yielding Malaysian rice variety, MR263, is susceptible to blast and was used as the recurrent parent in this study. To improve MR263 disease resistance, the Pongsu Seribu 1 rice variety was used as donor of the blast resistance Pi-7(t), Pi-d(t)1 and Pir2-3(t) genes and qLN2 quantitative trait locus (QTL). The objective was to introgress these blast resistance genes into the background of MR263 using marker-assisted backcrossing with both foreground and background selection. RESULTS: Improved MR263-BR-3-2, MR263-BR-4-3, MR263-BR-13-1 and MR263-BR-26-4 lines carrying the Pi-7(t), Pi-d(t)1 and Pir2-3(t) genes and qLN2 QTL were developed using the simple sequence repeat (SSR) markers RM5961 and RM263 (linked to the blast resistance genes and QTL) for foreground selection and a collection of 65 polymorphic SSR markers for background selection in backcrossed and selfed generations. A background analysis revealed that the highest rate of recurrent parent genome recovery was 96.1% in MR263-BR-4-3 and 94.3% in MR263-BR-3-2. CONCLUSION: The addition of blast resistance genes can be used to improve several Malaysian rice varieties to combat this major disease.
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Official URL or Download Paper: http://onlinelibrary.wiley.com/doi/10.1002/jsfa.72...
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Agriculture Faculty of Biotechnology and Biomolecular Sciences Institute of Tropical Agriculture |
DOI Number: | https://doi.org/10.1002/jsfa.7222 |
Publisher: | Society of Chemical Industry |
Keywords: | Blast resistance; Molecular markers; Pi gene; Rice |
Depositing User: | Nabilah Mustapa |
Date Deposited: | 19 May 2016 02:09 |
Last Modified: | 19 May 2016 02:09 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jsfa.7222 |
URI: | http://psasir.upm.edu.my/id/eprint/43399 |
Statistic Details: | View Download Statistic |
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