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Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning


Citation

Saallah, Suryani and Naim, Mohd Nazli and Lenggoro, Ignatius Wuled and Mokhtar, Mohd Noriznan and Abu Bakar, Noor Fitrah and Gen, Masao (2016) Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning. Biotechnology Reports, 10. pp. 44-48. ISSN 2215-017X

Abstract

Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
DOI Number: https://doi.org/10.1016/j.btre.2016.03.003
Publisher: Elsevier
Keywords: Nanofibres; Immobilisation; CGTase; Electrospinning; Cyclodextrins
Depositing User: Nabilah Mustapa
Date Deposited: 18 May 2016 04:11
Last Modified: 18 May 2016 04:11
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.btre.2016.03.003
URI: http://psasir.upm.edu.my/id/eprint/43211
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