Citation
Saallah, Suryani and Naim, Mohd Nazli and Lenggoro, Ignatius Wuled and Mokhtar, Mohd Noriznan and Abu Bakar, Noor Fitrah and Gen, Masao
(2016)
Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning.
Biotechnology Reports, 10.
pp. 44-48.
ISSN 2215-017X
Abstract
Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Engineering |
| DOI Number: | https://doi.org/10.1016/j.btre.2016.03.003 |
| Publisher: | Elsevier |
| Keywords: | Nanofibres; Immobilisation; CGTase; Electrospinning; Cyclodextrins |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 18 May 2016 04:11 |
| Last Modified: | 18 May 2016 04:11 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.btre.2016.03.003 |
| URI: | http://psasir.upm.edu.my/id/eprint/43211 |
| Statistic Details: | View Download Statistic |
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