Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)

Citation

Uda, Muhammad Nur Aiman and Che Man, Hasfalina and Abd Aziz, Samsuzana and Ishak, Zamri and Bunawan, Siti Noraini and Wahid, Nur Sabrina and Hashim, Uda (2014) Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV). Agriculture and Agricultural Science Procedia, 2. pp. 107-112. ISSN 2210-7843

Abstract

This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed with 5% Cellulases. Viral particles from both methods were examined by transmission electron microscopy (TEM) and rod-shaped with rounded ends was only seen on samples that were extracted with method 2. The estimated purity and concentration of method 2 is higher compare to method 1 which are 1.362 and 1.608 mg/ml and 0.958 and 0.299 mg/ml respectively.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Engineering
DOI Number:	https://doi.org/10.1016/j.aaspro.2014.11.016
Publisher:	Elsevier
Keywords:	Tungro disease; Rice tungro bacilliform virus (RTBV); Rice tungro spherical virus (RTSV)
Depositing User:	Mohd Noor Ismail
Date Deposited:	04 May 2016 07:08
Last Modified:	27 Sep 2017 08:56
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.aaspro.2014.11.016
URI:	http://psasir.upm.edu.my/id/eprint/43204
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