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Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site


Citation

Shahrestani, Kiarash Roohani and Ideris, Aini and Bejo, Mohd Hair and Tan, Sheau Wei and Yeap, Swee Keong and Rasoli, Mehdi and Jazayeri, Seyed Davoud and Goh, Su Hua and Omar, Abdul Rahman (2013) Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 117-119).

Abstract

Intensive vaccine programs have been implemented in many countries including Malaysia, but Newcastle disease virus (NDV) outbreaks have occurred, even in well-vaccinated farms. Hence, the present study was aimed to characterize five NDV isolates obtained from NDV vaccinated broiler farms in 2011 based on sequence and phylogenetic analysis of partial fusion gene. All the isolated NDV strains showed that they are categorized as velogenic NDV based on the presence of multi-basic protease cleavage sites, 112RRRKRF117. In addition, phylogenetic analysis showed that the isolates can classified under the genotype VII, subgenotype VIId.


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Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Faculty of Veterinary Medicine, Universiti Putra Malaysia
Keywords: Newcastle Disease Virus (NDV); Genotyping; Phylogenetic Analysis
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Nov 2015 06:54
Last Modified: 28 Jan 2016 01:54
URI: http://psasir.upm.edu.my/id/eprint/41411
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