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Phylogenetic analysis of haemaglutinin gene of avian influenza virus isolate VRI1803/04


Citation

Zulkifli, Hazalina and Ideris, Aini and Omar, Abdul Rahman and Syed Hassan, Sharifah (2013) Phylogenetic analysis of haemaglutinin gene of avian influenza virus isolate VRI1803/04. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 113-114).

Abstract

An isolate of avian influenza virus (AIV) designated as VRI1803/04 obtained from Veterinary Research Institute, Ipoh, Perak was used for identification and characterisation using the conventional one step reverse transcription polymerase chain reaction (RT-PCR). Total RNA of the virus was obtained from allantoic fluid of specific-pathogen-free (SPF) embryonated chicken eggs extracted using conventional method. The complete sequence of H3 gene (1701 bp) of VRI1803/04 was determined. The H gene has 89% homology to H3 gene from A/duck/Ukraine/1/63 (H3N8).


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Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Faculty of Veterinary Medicine
Publisher: Faculty of Veterinary Medicine, Universiti Putra Malaysia
Keywords: Avian influenza virus; Nucleotide Sequencing; Allantoic fluid; Polymerase chain reaction; Reverse transcription
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Nov 2015 06:36
Last Modified: 28 Jan 2016 01:51
URI: http://psasir.upm.edu.my/id/eprint/41409
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