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Detection of mycoplasma gallisepticum by real time PCR using gapA gene


Citation

Yasmin, Farhana and Ideris, Aini and Omar, Abdul Rahman and Bejo, Mohd Hair and Tan, Sheau Wei and Tan, Ching Giap and Islam, R. and Ahmad, K. (2013) Detection of mycoplasma gallisepticum by real time PCR using gapA gene. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 107-108).

Abstract

Mycoplasma gallisepticum (MG) is responsible for chronic respiratory disease which leads to a lot of economic losses to the poultry industry. Therefore, it is very important to detect this organism early and efficiently. MG can be diagnosed by three ways: isolation identification, serological method and molecular detection method. In this study, a SYBR green real time PCR assay by using gapA gene was developed for the detection of MG. This assay was powerful both from the aspect of specificity and sensitivity as it amplified only MG at early cycle when run together with 20 other avian Mycoplasma species and it can detect a minimum of 26 pg/μl of DNA template.


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Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Faculty of Veterinary Medicine, Universiti Putra Malaysia
Keywords: Mycoplasma gallisepticum; PCR; SYBR green real time PCR; GapA gene
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Nov 2015 06:14
Last Modified: 28 Jan 2016 01:07
URI: http://psasir.upm.edu.my/id/eprint/41404
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