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Molecular quantitation and characterization of Vibrio cholerae from different seafood obtained from wetmarket and supermarket


Citation

Letchumanan, Vengadesh and Radu, Son and Cheah, Yoke Kqueen (2012) Molecular quantitation and characterization of Vibrio cholerae from different seafood obtained from wetmarket and supermarket. International Food Research Journal, 19 (1). pp. 45-50. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Vibrio cholerae still represents a significant threat to human health worldwide despite the advances in hygiene, consumer knowledge, food treatment and food processing. In Malaysia, statistics in year 2009 have shown that among the food and water borne diseases, food poisoning has the highest incidence rate of 36.17 per 100,000 populations and with a mortality rate of 0.01 per 100,000 populations. In this study, 22 seafood samples comprising of fish, squid, crustacean and mollusks purchased from wet market and supermarket were analyzed. The Most Probable Number (MPN) and real time PCR was used to enumerate the Vibrio cholerae in seafood sample. The results showed that MPN-real time PCR of the samples from wet market had a maximum of > 1100 MPN/g compare to 93 MPN/g enumerated from the MPN plate. The MPN-real time PCR in the samples from supermarket indicated 290 MPN/g as compared to 240 MPN/g enumerated from the MPN plate. The standard curves showed that there was a good linear correlation between the Ct values. The minimum level of detection of Vibrio cholerae standard DNA at targeted gene was 3 × 10 -5 ng/μl.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Most probable number (MPN); Real time PCR; Seafood; Supermarket; Vibrio cholerae; Wet market
Depositing User: Najah Mohd Ali
Date Deposited: 30 Nov 2015 07:37
Last Modified: 29 Aug 2017 09:08
URI: http://psasir.upm.edu.my/id/eprint/41048
Statistic Details: View Download Statistic

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