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Optimization of multiplex PCR conditions for rapid detection of Escherichia coli O157:H7 virulence genes


Citation

Jeshveen S. S., and Chai, Lay Ching and Pui, Chai Fung and Radu, Son (2012) Optimization of multiplex PCR conditions for rapid detection of Escherichia coli O157:H7 virulence genes. International Food Research Journal, 19 (2). pp. 461-466. ISSN 1985-4668; ESSN: 2231-7546

Abstract / Synopsis

The main source of E. coli 0157:H7 is cattle, but recent studies showed high percentage of outbreaks contributed by contaminated water. The occurrence of E. coli O157:H7 in environmental water samples poses a potential threat to human health. The aim of this study was to establish a protocol for the detection of the pathogen E. coli O157:H7 and E. coli virulence genes (eaeA, rfbE, hly, stx 1, and stx 2) in a multiplex PCR protocol using six specific primer pairs. The target genes produced species-specific amplicons at 625 bp, 397 bp, 296 bp, 166 bp, 210 bp and 484 bp for E. coli O157:H7 (fliC h7 gene) and virulence genes (eaeA, rfbE, hly, stx 1, and stx 2) respectively. The results obtained show that the established PCR protocol is suitable for a rapid and specific analysis of the pathogenic E. coli O157:H7 in environmental water samples for the assessment of microbiological risks.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Escherichia coli O157:H7; Multiplex PCR; Optimization
Depositing User: Najah Mohd Ali
Date Deposited: 30 Nov 2015 13:09
Last Modified: 30 Nov 2015 13:09
URI: http://psasir.upm.edu.my/id/eprint/41042
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