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Optimization of multiplex PCR conditions for rapid detection of Escherichia coli O157:H7 virulence genes


Citation

Ranjit Singh, Jeshveen Singh and Chai, Lay Ching and Pui, Chai Fung and Radu, Son (2012) Optimization of multiplex PCR conditions for rapid detection of Escherichia coli O157:H7 virulence genes. International Food Research Journal, 19 (2). pp. 461-466. ISSN 1985-4668; ESSN: 2231-7546

Abstract

The main source of E. coli 0157:H7 is cattle, but recent studies showed high percentage of outbreaks contributed by contaminated water. The occurrence of E. coli O157:H7 in environmental water samples poses a potential threat to human health. The aim of this study was to establish a protocol for the detection of the pathogen E. coli O157:H7 and E. coli virulence genes (eaeA, rfbE, hly, stx 1, and stx 2) in a multiplex PCR protocol using six specific primer pairs. The target genes produced species-specific amplicons at 625 bp, 397 bp, 296 bp, 166 bp, 210 bp and 484 bp for E. coli O157:H7 (fliC h7 gene) and virulence genes (eaeA, rfbE, hly, stx 1, and stx 2) respectively. The results obtained show that the established PCR protocol is suitable for a rapid and specific analysis of the pathogenic E. coli O157:H7 in environmental water samples for the assessment of microbiological risks.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Escherichia coli O157:H7; Multiplex PCR; Optimization
Depositing User: Najah Mohd Ali
Date Deposited: 30 Nov 2015 05:09
Last Modified: 14 Jul 2020 04:46
URI: http://psasir.upm.edu.my/id/eprint/41042
Statistic Details: View Download Statistic

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