Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism

Citation

Chai, Lay Ching and Abdullah, Fatimah Corazon and Mat Sout, Norhisyam and Alias, Rozila and Abu Samah, Nadzirah and Lee, Natasha Hai Yen (2009) Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism. International Food Research Journal, 16 (1). pp. 113-118. ISSN 1985-4668; ESSN: 2231-7546

Abstract

The objective of the present study was to develop a rapid, reliable and yet inexpensive protocol for genomic DNA extraction from frozen and ethanol-preserved Asian green-lipped mussels for random amplified microsatelite (RAM) analysis. The procedure comprised of three major steps: (1) Tissue degradation by boiling in 6% Chelex 100 resin in TE buffer; (2) Protein digestion by Proteinase K; and (3) DNA precipitation by adding 2 volumes of cold absolute ethanol. The entire procedure can be completed within two hours. The resulting RAM profiles were clear and reproducible. Our results demonstrate that the combined protocol of Chelex 100- Proteinase K-ethanol precipitation is a powerful yet economical DNA isolation method for population genetic studies involving a large sample size.

Download File

Preview

PDF
15 IFRJ-2008-140 Chai Malaysia FINAL.pdf
Download (474kB) | Preview

Official URL or Download Paper: http://www.ifrj.upm.edu.my/16%20%281%29%202009/15%...

Additional Metadata

Item Type:	Article
Divisions:	Faculty of Food Science and Technology
Publisher:	Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords:	Chelex 100 resin; DNA extraction; Mussel; RAM
Depositing User:	Nabilah Mustapa
Date Deposited:	09 Oct 2015 05:02
Last Modified:	09 Oct 2015 05:02
URI:	http://psasir.upm.edu.my/id/eprint/40902
Statistic Details:	View Download Statistic

Actions (login required)

View Item