Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis

Citation

Lihan, Samuel and Mobilik, Marian Madonna and Apun, Kasing and Bilung, Lesley Maurice and Radu, Son (2011) Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis. International Food Research Journal, 18 (3). pp. 971-976. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their surrounding environment were determined. The levels of resistance of the E. coli isolates towards six different antibiotics tested differed considerably. Though the isolates displayed resistance towards some of the antibiotics tested, none of the isolates showed resistant towards norfloxacin, sulphametoxazole/trimethoprim and chloramphenicol. RAPD-PCR analysis using single primer and primers combination clustered the E. coli isolates into 3 and 5 groups, respectively. The results of this study suggest that the E. coli isolates from the catfish and their surrounding environment derived from a mixture of sensitive and resistant strains with diverse genetic contents. The use of the RAPD analysis is sufficiently discriminatory for the typing of the E. coli isolates.

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Official URL or Download Paper: http://www.ifrj.upm.edu.my/18%20%2803%29%202011/%2...

Additional Metadata

Item Type:	Article
Divisions:	Faculty of Food Science and Technology
Publisher:	Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords:	Antibiotic resistance; E. coli; Genetic diversity; RAPD-PCR
Depositing User:	Najah Mohd Ali
Date Deposited:	27 Nov 2015 03:07
Last Modified:	24 Oct 2019 03:51
URI:	http://psasir.upm.edu.my/id/eprint/40682
Statistic Details:	View Download Statistic

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