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Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)


Citation

Lee, C. W. and Tan, C. H. (1983) Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 74 (4). pp. 791-792. ISSN 0305-0491

Abstract

1. The activities of hexokinase and carnitine palmitoyltransferase (CPT) activities were assayed in flight muscle mitochondria of Valanga nigricornis. 2. Kinetic analysis of the enzyme activities yielded the following information: for hexokinase, its Km (glucose) is 150 μM while its Vmax is 2.5 μmol/min/mg protein; the corresponding data for CPT are Km (palmitoylCoA) of 39 μM and Vmax of 22.2 nmol/min/mg mitochondrial protein. 3. Under conditions of equivalent rates of ATP production the hexokinase-CPT activity ratio is 64, suggesting that although V. nigricornis is a “mixed” fuel utilizer, it appears that carbohydrate oxidation constitutes the predominant energy source.


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Additional Metadata

Item Type: Article
Divisions: Universiti Pertanian Malaysia
DOI Number: https://doi.org/10.1016/0305-0491(83)90146-3
Publisher: Pergamon Press
Keywords: Hexokinase; Carnitine palmitoyltransferase; Valanga nigricornis
Depositing User: Nabilah Mustapa
Date Deposited: 18 Sep 2015 04:59
Last Modified: 15 Oct 2024 06:36
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/0305-0491(83)90146-3
URI: http://psasir.upm.edu.my/id/eprint/40470
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